5'-Deoxy-5'-Fluoro-Thymidine, a Biochemical Analogue of Thymidine-5'-Monophosphate Selectively Inhibiting DNA Synthesis
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منابع مشابه
Fractionation of Thymidine Phosphokinase, Thymidine 5'-monophosphate Phosphokinase and Thymidine 5'-diphosphate Phosphokinase in Extracts of Landschutz Ascites-tumour Cells.
1. Extracts of Landschutz ascites-tumour cells have been fractionated by treatment with acid, alumina C(gamma) gel and Sephadex G-100 to yield purified preparations of thymidine phosphokinase, thymidine 5'-monophosphate phosphokinase and thymidine 5'-diphosphate phosphokinase. 2. These results clearly demonstrate the existence in Landschutz ascites tumour of three phosphokinases each of which c...
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5-Florouracil (5-FU) is the basic agent used in the treatment of gastric cancer. Capecitabine, a prodrug of 5-FU, displays increased antitumor efficacy compared with 5-FU in the clinic. 5'-Deoxy-5-fluorouracil (5'-DFUR), the metabolite of capecitabine, is converted to 5-FU by the enzyme thymidine phosphorylase (TP), which is present at high concentrations in human tumors. In this study, we inve...
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[formula: see text] 5-Amino-2'-deoxyuridine 5'-triphosphate, an analogue of deoxythymidine triphosphate, was synthesized and found to be a substrate of Taq DNA polymerase. The DNA-borne analogue underwent selective chemical reaction with permanganate. The use of 5-amino-dU as an interference probe was validated using the Ada protein/ada promoter complex. The performance of 5-amino-dU in interfe...
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The substitution of a hydroxyl group by a fluorine atom in a potential drug is an efficient reaction that can, in principle, improve its pharmacological properties. Herein, the synthesis of the novel compound 5'-fluoro-5'-deoxyacadesine (5'-F-AICAR), a strict analogue of AICAR that cannot be 5'-phosphorylated to ZMP by cellular kinases, is reported.
متن کاملPurification and characterization of Thymidine 5-monophosphate kinase from Escherichia coli B.
Thymidylate kinase was purified about SOOO-fold from Eschenkhia coti B. The final steps in the purification procedure utilized preparative disc gel electrophoresis, and the most highly purified preparation appeared to be homogeneous with respect to particle size, sedimentation velocity, and electrophoretic mobility. The enzyme phosphorylated deoxythymidine monophosphate, 5-iodo-2’-deoxyuridylic...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1968
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1968.tb00454.x